LOCUS       40924_23364       12650 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB580omega DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Nakagawa T, Tanaka Y, Kimura T, Ishiguro S.
  TITLE     Gateway binary vector pGWB for RNAi
  JOURNAL   Published Only in Database (2012)
REFERENCE   2  (bases 1 to 12650)
  AUTHORS   Nakagawa T, Tanaka Y, Kimura T, Ishiguro S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12650)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12650)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1091..1157
                     /label=omega translational enhancer
                     /note="omega translational enhancer"
     protein_bind    1187..1311
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1336..1366
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1718..2020
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2064..2188)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2211..2565
                     /label=ICDH 1st intron (AT1G65930)
                     /note="ICDH 1st intron (AT1G65930)"
     protein_bind    2586..2710
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2754..3056)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3413..3537)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3571..3823
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3857..3873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3881..3897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3905..3935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3950..3971)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4007..4259)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4413..5435)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5487..5666)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6172..6196)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6717..7505
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7754..8342
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8528..8668)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9012..9206)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9275..10345)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10777..11403)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gtactcgaga tcctctagag ttatcaacaa gtttgtacaa
     1201 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     1261 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca
     1321 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     1381 taggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     1441 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa
     1501 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     1561 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     1621 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     1681 aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta
     1741 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     1801 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     1861 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     1921 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     1981 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     2041 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     2101 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     2161 ctcgttcagc tttcttgtac aaagtggttg ataacagcgc ttaactagtg gtgagatctt
     2221 acttctcctt ctcctctctc gatctctttc gctatctata atcagatatt ttcgttgatt
     2281 ggatacgaaa tgatttacct tcgcttgcct agattgtaac gaagtttcgt tgtttgttct
     2341 gatctgatct ctggaatcta cgcatgtgta aactcgttga ttgatttggt tttggtgtat
     2401 gattacgtta tagatcgata aaatcgataa aacctttaag ctctgttcat gagattgatt
     2461 gatttatgaa aaatctggac tcgatttcaa gtgtggattt gatttacagc tttgattcgg
     2521 cgaaatagtt tcgatattta agcttcattg gtttgtgtaa tgtaggtacc taagcgctgt
     2581 tatcaaccac tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata
     2641 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
     2701 agtcactatg gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
     2761 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
     2821 tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt
     2881 tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt
     2941 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
     3001 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
     3061 ccagtccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc
     3121 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
     3181 catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag caactgatag
     3241 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag cacacctctt tttgacatac
     3301 ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat
     3361 actgttatct ggcttttagt aagccggatc cagatctcga tcgaggggcc gccatagtga
     3421 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
     3481 tatattgata tttatatcat tttacgtttc tcgttcagct tttttgtaca aacttgttga
     3541 taactctaga ggatgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     3601 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     3661 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     3721 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     3781 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa
     3841 ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3961 aactcacatt aggctgaatt aggcgcgcct atttctatta attcccgatc tagtaacata
     4021 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     4081 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     4141 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     4201 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg
     4261 gggaaattcg ggggatcgat cccgcgaact gtggacgaga actgtgccac caagcgtaag
     4321 gccgttctct cgcatttgcc ttgctaggct cgcgcgagtt gctggctgag gcgttctcga
     4381 aatcagctct tgttcggtcg gcatctactc tattcctttg ccctcggacg agtgctgggg
     4441 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     4501 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     4561 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     4621 tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
     4681 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     4741 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     4801 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     4861 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     4921 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     4981 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     5041 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
     5101 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     5161 gagatgcaat aggtcaggct ctcgctgaat gccccaatgt caagcacttc cggaatcggg
     5221 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     5281 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     5341 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
     5401 tcgacagacg tcgcggtgag ttcaggcttt ttcatatctt attgcccccc gggatcagat
     5461 ctggattgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     5521 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     5581 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     5641 gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     5701 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatct tgatcccctg
     5761 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
     5821 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag
     5881 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt
     5941 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
     6001 ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag
     6061 cgtgaagctg atccgtcgag attaatagaa ataaattcag cctaattcgg cgttaattca
     6121 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     6181 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     6241 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     6301 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     6361 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     6421 gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     6481 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     6541 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     6601 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     6661 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
     6721 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     6781 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     6841 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     6901 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     6961 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     7021 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     7081 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     7141 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     7201 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     7261 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     7321 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     7381 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     7441 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     7501 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     7561 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     7621 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
     7681 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7741 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7801 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7861 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     7921 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7981 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8041 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8101 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8161 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8221 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8281 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8341 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8401 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8461 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8521 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     8581 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     8641 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     8701 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     8761 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     8821 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     8881 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     8941 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     9001 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     9061 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     9121 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     9181 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
     9241 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     9301 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     9361 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     9421 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     9481 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
     9541 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
     9601 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
     9661 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
     9721 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
     9781 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
     9841 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
     9901 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
     9961 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
    10021 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
    10081 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
    10141 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    10201 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    10261 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    10321 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
    10381 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
    10441 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
    10501 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
    10561 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
    10621 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
    10681 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
    10741 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
    10801 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
    10861 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
    10921 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
    10981 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
    11041 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
    11101 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
    11161 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
    11221 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
    11281 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
    11341 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
    11401 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
    11461 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
    11521 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
    11581 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
    11641 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
    11701 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
    11761 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
    11821 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
    11881 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
    11941 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
    12001 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
    12061 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
    12121 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
    12181 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
    12241 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
    12301 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
    12361 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    12421 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    12481 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    12541 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag
    12601 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
